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Image Search Results
Journal: CNS Neuroscience & Therapeutics
Article Title: Orexin‐A Attenuates the Inflammatory Response in Sepsis‐Associated Encephalopathy by Modulating Oxidative Stress and Inhibiting the ERK / NF ‐ κB Signaling Pathway in Microglia and Astrocytes
doi: 10.1111/cns.70096
Figure Lengend Snippet: OXA affects the activation of hippocampal type A1/A2 reactive astrocytes. OXA treatment decreases the number of type A1 astrocytes and increases the number of type A2 astrocytes. (A) Representative immunofluorescence images showing the co‐localization of GFAP‐labeled astrocytes with c3‐labeled A1 astrocytes and the co‐localization of GFAP‐labeled astrocytes with S100A10‐labeled A2 astrocytes co‐localized in the hippocampus. Scale bar = 20 μm. (B) Percentage of c3‐positive cells in the hippocampus that are GFAP‐positive. (C) Percentage of S100A10 positive cells in the hippocampal region that are GFAP positive. One‐way ANOVA and Tukey's post hoc test were used. The values were presented as mean ± SD (* p < 0.05, ** p < 0.01, # p < 0.0001, n = 3 for each group).
Article Snippet: The primary antibodies used were: mouse anti‐Iba‐1 (GB123502‐100, Servicebio), mouse anti‐GFAP (#3670, Cell Signaling Technology), rabbit anti‐Iba‐1 (EPR16588, Abcam), rabbit anti‐P‐ERK (#9926, Cell Signaling Technology), rabbit anti‐NF‐κB (SZ10‐04, HuaBio), rabbit anti‐C3 (ET1702‐99, HuaBio), rabbit
Techniques: Activation Assay, Immunofluorescence, Labeling